TARGETED SECRETION OF CUTINASE IN FUSARIUM-SOLANI F-SP PISI AND COLLETOTRICHUM-GLOEOSPORIOIDES

被引：21

作者：

PODILA, GK ^{[1
]}

ROSEN, E ^{[1
]}

SANFRANCISCO, MJD ^{[1
]}

KOLATTUKUDY, PE ^{[1
]}

机构：

[1] OHIO STATE UNIV,CTR BIOTECHNOL,COLUMBUS,OH 43210

来源：

PHYTOPATHOLOGY | 1995年 / 85卷 / 02期

关键词：

D O I：

10.1094/Phyto-85-238

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Immunofluorescence studies of cutinase secretion by phytopathogenic fungi Fusarium solani f. sp. pisi and Colletotrichum gloeosporioides were made to determine targeting of the enzyme in germinating spores. Our results indicate that the secretion of cutinase was directed towards the region that penetrates the host. In F. s. pisi, which penetrates plant tissue without appressorium formation, cutinase was targeted to the growing tip of germinating spores. In C. gloeosporioides, cutinase secretion was directed to the infection peg that arises from the appressorium. Monensin, the carboxylic ionophore, inhibited secretion of cutinase and caused intracellular accumulation of cutinase in F. s. pisi. Subcellular fractionation of monensin-treated cells indicated involvement of the Golgi in the secretion and targeting of cutinase by germinating spores of F. s. pisi.

引用

页码：238 / 242

页数：5

共 30 条

[1]

CALVETE JS, 1992, THESIS U UTRECHT NET, P75

[2]

Collmer A., 1991, Advances in molecular genetics of plant-microbe interactions. Vol. 1. Proceedings of the 5th international symposium on the molecular genetics of plant-microbe interactions, Interlaken, Switzerland, September 9-14, 1990., P65

[3] PECTATE LYASE FROM FUSARIUM-SOLANI F SP-PISI - PURIFICATION, CHARACTERIZATION, INVITRO TRANSLATION OF THE MESSENGER-RNA, AND INVOLVEMENT IN PATHOGENICITY [J].