EVIDENCE FOR A HEPATOCYTE MEMBRANE FATTY-ACID TRANSPORT PROTEIN USING RAT-LIVER MESSENGER-RNA EXPRESSION IN XENOPUS-LAEVIS OOCYTES

被引：16

作者：

FITSCHER, BA ^{[1
]}

KLAASSENSCHLUTER, CM ^{[1
]}

STREMMEL, W ^{[1
]}

机构：

[1] UNIV HEIDELBERG HOSP,DEPT MED 4,D-69115 HEIDELBERG,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1995年 / 1256卷 / 01期

关键词：

FATTY ACID UPTAKE; EXPRESSION CLONING; (XENOPUS LAEVIS OOCYTE);

D O I：

10.1016/0005-2760(95)00003-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to directly demonstrate that hepatocellular uptake of long-chain fatty acids represents a non-diffusional uptake mechanism. Xenopus laevis oocytes were used for expression of rat liver mRNA to identify the liver fatty acid uptake system. Injection of total rat liver poly(A)(+) RNA into oocytes resulted in a dose-dependent increase in fatty acid uptake. The most active mRNA was found in the 1.1-2.1 kb subfraction. In contrast, expression of the liver cytosolic fatty acid binding protein (L-FABP) or the previously suggested candidate carrier protein, mitochondrial aspartate aminotransferase (mGOT), did not induce fatty acid uptake. It is concluded that in rat liver, fatty acid transport represents a protein-mediated transport system.

引用

页码：47 / 51

页数：5

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