HUMAN GENES FOR THE ALPHA-CHAIN AND BETA-CHAIN OF COMPLEMENT C4B-BINDING PROTEIN ARE CLOSELY LINKED IN A HEAD-TO-TAIL ARRANGEMENT

被引：59

作者：

PARDOMANUEL, F ^{[1
]}

REYCAMPOS, J ^{[1
]}

HILLARP, A ^{[1
]}

DAHLBACK, B ^{[1
]}

DECORDOBA, SR ^{[1
]}

机构：

[1] UNIV LUND,MALMO GEN HOSP,DEPT CLIN CHEM,S-21401 MALMO,SWEDEN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 12期

关键词：

physical linkage; regulator of complement activation gene cluster;

D O I：

10.1073/pnas.87.12.4529

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

C4b-binding protein (C4BP) is an important component in the regulation of the complement system and also binds the anticoagulant vitamin K-dependent protein S. These activities are performed by distinct, although structurally related, polypeptides of 70 kDa (α chain) and 45 kDa (β chain), respectively. In this report we have investigated the genetic relationships between these polypeptides. Using pulsed field gel electrophoresis analysis we demonstrate that the genes coding for the α (C4BPα) and β (C4BPβ) chains are closely linked within the regulator of complement activation gene cluster. In addition, we have determined that the 3' end of the C4BPβ gene lies 3.5-5 kilobases from the 5' end of the C4BPα gene. These findings support the concept that the C4BPα and C4BPβ genes are the result of a gene duplication event.

引用

页码：4529 / 4532

页数：4

共 30 条

[1] STRUCTURAL GENE FOR HUMAN MEMBRANE COFACTOR PROTEIN (MCP) OF COMPLEMENT MAPS TO WITHIN 100-KB OF THE 3' END OF THE C3B/C4B RECEPTOR GENE [J].

BORA, NS ;

LUBLIN, DM ;

KUMAR, BV ;

HOCKETT, RD ;

HOLERS, VM ;

ATKINSON, JP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (02) :597-602

[2] CLONING OF DECAY-ACCELERATING FACTOR SUGGESTS NOVEL USE OF SPLICING TO GENERATE 2 PROTEINS [J].

CARAS, IW ;

DAVITZ, MA ;

RHEE, L ;

WEDDELL, G ;

MARTIN, DW ;

NUSSENZWEIG, V .

NATURE, 1987, 325 (6104) :545-549

[3] ORGANIZATION OF THE GENES ENCODING COMPLEMENT RECEPTORS TYPE-1 AND TYPE-2, DECAY-ACCELERATING FACTOR, AND C4-BINDING PROTEIN IN THE RCA LOCUS ON HUMAN CHROMOSOME-1 [J].

CARROLL, MC ;

ALICOT, EM ;

KATZMAN, PJ ;

KLICKSTEIN, LB ;

SMITH, JA ;

FEARON, DT .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (04) :1271-1280

[4] SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].

CHU, G ;

VOLLRATH, D ;

DAVIS, RW .

SCIENCE, 1986, 234 (4783) :1582-1585

[5] MOLECULAR-CLONING AND CHARACTERIZATION OF THE CDNA CODING FOR C4B-BINDING PROTEIN, A REGULATORY PROTEIN OF THE CLASSICAL PATHWAY OF THE HUMAN-COMPLEMENT SYSTEM [J].

CHUNG, LP ;

BENTLEY, DR ;

REID, KBM .

BIOCHEMICAL JOURNAL, 1985, 230 (01) :133-141

[6] VISUALIZATION OF HUMAN C4B-BINDING PROTEIN AND ITS COMPLEXES WITH VITAMIN-K-DEPENDENT PROTEIN-S AND COMPLEMENT PROTEIN-C4B [J].

DAHLBACK, B ;

SMITH, CA ;

MULLEREBERHARD, HJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (11) :3461-3465

[7] HUMAN GENES FOR 3 COMPLEMENT COMPONENTS THAT REGULATE THE ACTIVATION OF C-3 ARE TIGHTLY LINKED [J].

DECORDOBA, SR ;

LUBLIN, DM ;

RUBINSTEIN, P ;

ATKINSON, JP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1985, 161 (05) :1189-1195

[8] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[9] ROLE OF C4-BINDING PROTEIN AND BETA-1H IN PROTEOLYSIS OF C4B AND C3B [J].

FUJITA, T ;

NUSSENZWEIG, V .

JOURNAL OF EXPERIMENTAL MEDICINE, 1979, 150 (02) :267-276

[10] HUMAN C4-BINDING PROTEIN .2. ROLE IN PROTEOLYSIS OF C4B BY C3B-INACTIVATOR [J].

FUJITA, T ;

GIGLI, I ;

NUSSENZWEIG, V .

JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 148 (04) :1044-1051

← 1 2 3 →