STRINGENT SPACING REQUIREMENTS FOR TRANSCRIPTION ACTIVATION BY CRP

被引：244

作者：

GASTON, K ^{[1
]}

BELL, A ^{[1
]}

KOLB, A ^{[1
]}

BUC, H ^{[1
]}

BUSBY, S ^{[1
]}

机构：

[1] UNIV BIRMINGHAM,SCH BIOCHEM,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND

来源：

CELL | 1990年 / 62卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0092-8674(90)90118-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cyclic AMP receptor protein-cAMP complex (CRP-cAMP) binds at a variety of distances upstream of several E. coli promoters and activates transcription. We have constructed a model system in which a consensus CRP binding site is placed at different distances upstream of the melR promoter. CRP-cAMP activates transcription from melR when bound at a number of positions, all of which lié on the same face of the DNA helix. The two distances at which transcription is strongly activated correspond exactly to those at which CRP-cAMP binds upstream of the well-studied galP1 and lac promoters. Footprinting of the synthetic promoters reveals that RNA polymerase makes identical contacts with their -10 regions even though CRP-cAMP binds at a different distance in each case. Kinetic analysis in vitro indicates that CRP-cAMP activates transcription from these promoters in similar but distinct ways. A model is proposed to explain this two-position activation. © 1990.

引用

页码：733 / 743

页数：11

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