HIGH-PERFORMANCE EXCLUSION CHROMATOGRAPHY OF WATER-SOLUBLE POLYMERS WITH CHEMICALLY BONDED STATIONARY PHASES

Chemically bonded stationary phases with 14 different functional groups have been evaluated for use in the exclusion chromatography of water-soluble polymers. They were characterized by their interaction with different polyethylene glycol standards. A bonded glycinamide and a diamine phase showed no interaction with polyethylene glycols and such phases bonded to silicas having different pore diameter distribution are suitable for exclusion chromatography. A bonded amide phase proved to be more suitable for exclusion chromatography of polyvinyl pyrrollidones, dextrams and gelatine but partially retained the polyethylene glycols. For the separation of polymers in the molecular-weight range 100-70,000 the average pore diameter of the silica matrix should be at least 200 Å; for those in the molecular-weight range 80,000-500,000 the pore diameter should be ca. 500 Å. © 1979.