DEXTRAN MODIFIED SELF-ASSEMBLED MONOLAYER SURFACES FOR USE IN BIOINTERACTION ANALYSIS WITH SURFACE-PLASMON RESONANCE

The development of a surface modification method based on self-assembled monolayers on gold surfaces has proved to be a successful basis for the functionalization of sensor surfaces intended for use in biospecific interaction analysis. The dextran coated alkanethiol layer shows high chemical stability towards various conditions employed for different analytical sequences. Optimized synthesis sequences yield highly reproducible sensor surfaces in terms of surface coverage and biomolecule binding capacities. Immobilization chemistries adapted for coupling various biomolecules and other ligands were developed for the dextran coated surface and used in bioanalysis techniques based on surface plasmon resonance. This mass sensitive, real-time analysis technique with label-free detection has developed as a universal tool for analysis of various qualitative and quantitative biospecific interactions, including affinity and kinetic rate constant determinations.