SEQUENCES OF HEAVY AND LIGHT-CHAIN VARIABLE REGIONS FROM 4 BOVINE IMMUNOGLOBULINS

Oligodeoxyribonucleotide primers based on the 5' ends of bovine IgG1/2 and lambda constant (C) region genes, together with primers encoding conserved amino acids at the N-terminus of mature variable (V) regions from other species, have been used in cDNA and polymerase chain reactions (PCRs) to amplify heavy and light chain V region cDNA from bovine heterohybridomas. The amino acid sequences of V-H and V-lambda from four bovine immunoglobulins of different specificities are presented.