LINEAR DICHROISM OF RHODOPSIN IN AIR-WATER-INTERFACE FILMS

被引:11
作者
KORENBROT, JI [1 ]
JONES, O [1 ]
机构
[1] UNIV CALIF SAN FRANCISCO,SCH MED,DEPT BIOCHEM,SAN FRANCISCO,CA 94143
关键词
D O I
10.1007/BF01868766
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Air-water interface films of purified cattle rhodopsin and defined phospholipids are formed by the osmotic lysis of reconstituted membrane vesicles. The interface films thus formed consist of a phospholipid monolayer containing vesicle membrane fragments. Rhodopsin molecules at the interface are restricted within the membrane fragments where they are spectrophotometrically intact and capable of undergoing photoregeneration and chemical regeneration. Multilayers of up to 8 layers can be built from these interface films. The visible absorption band of rhodopsin in these multilayers is linearly dichroic. Quantitative analysis of the linear dichroism reveals that the dipole moment of transition of the retinal chromophore in rhodopsin forms an angle of 15°±4° with the plane of the membrane fragments in the interface film. This orientation of the chromophore relative to the plane of the membrane is essentially the same as that observed in the intact retina. Thus, the orientation of rhodopsin in the interface films is similar to that in the intact disc membranes. © 1979 Springer-Verlag New York Inc.
引用
收藏
页码:239 / 254
页数:16
相关论文
共 48 条
[1]   RHODOPSIN - PURIFICATION AND RECOMBINATION WITH PHOSPHOLIPIDS ASSAYED BY METARHODOPSIN I -] METARHODOPSIN II TRANSITION [J].
APPLEBURY, ML ;
ZUCKERMAN, DM ;
LAMOLA, AA ;
JOVIN, TM .
BIOCHEMISTRY, 1974, 13 (17) :3448-3458
[2]   SATURATION TRASFER ELECTRON-PARAMAGNETIC RESONANCE ON MEMBRANE-BOUND PROTEINS .1. ROTATIONAL DIFFUSION OF RHODOPSIN IN VISUAL RECEPTOR MEMBRANE [J].
BAROIN, A ;
THOMAS, DD ;
OSBORNE, B ;
DEVAUX, PF .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 78 (01) :442-447
[3]   RHODOPSIN IN ROD OUTER SEGMENT PLASMA-MEMBRANE [J].
BASINGER, S ;
BOK, D ;
HALL, M .
JOURNAL OF CELL BIOLOGY, 1976, 69 (01) :29-42
[4]   STRUCTURE OF FROG PHOTORECEPTOR MEMBRANES [J].
BLAUROCK, AE ;
WILKINS, MHF .
NATURE, 1969, 223 (5209) :906-&
[5]   Built-up films of barium stearate and their optical properties [J].
Blodgett, KB ;
Langmuir, I .
PHYSICAL REVIEW, 1937, 51 (11) :0964-0982
[6]  
BOGOMOLNI RA, 1977, BIOPHYS J, V17, pA98
[7]   RHODOPSIN ROTATES IN VISUAL RECEPTOR MEMBRANE [J].
BROWN, PK .
NATURE-NEW BIOLOGY, 1972, 236 (63) :35-&
[8]   POLARIZED ABSORPTION SPECTROSCOPY OF CHLOROPHYLL-LIPID MEMBRANES [J].
CHERRY, RJ ;
CHAPMAN, D ;
HSU, K .
BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 267 (03) :512-&
[9]   VERTEBRATE RETINAL CELLS AND THEIR ORGANIZATION [J].
COHEN, AI .
BIOLOGICAL REVIEWS, 1963, 38 (04) :427-+
[10]  
CONE RA, 1967, SCIENCE, V156, P536