NUCLEOTIDE-SEQUENCE OF DIHYDROFOLATE-REDUCTASE TYPE-VI

被引：19

作者：

WYLIE, BA

KOORNHOF, HJ

机构：

[1] UNIV WITWATERSRAND, MRC, EMERGENT PATHOGEN UNIT, JOHANNESBURG 2001, SOUTH AFRICA

[2] S AFRICAN INST MED RES, JOHANNESBURG 2000, SOUTH AFRICA

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1991年 / 35卷 / 04期

关键词：

D O I：

10.1099/00222615-35-4-214

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete sequence of the type VI dihydrofolate reductase (DHFR) gene from plasmid pUK672 was determined. The structural gene coded for a polypeptide of 157 amino acids which had a deduced mol. wt of 17 424. Comparison with amino-acid sequences of the type I, type V and Escherichia coli K12 chromosomal DHFRs showed that there was 63%, 61% and 31% homology respectively. Putative RNA polymerase and ribosomal binding sites were identified proximal to the initiation codon and a feature consistent with transcription termination was present distal to the coding region. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed that the enzyme had a subunit mol. wt of 17 500.

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页码：214 / 218

页数：5

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