CONFORMATIONAL RIGIDITY OF N4-ACETYL-2'-O-METHYLCYTIDINE FOUND IN TRANSFER-RNA OF EXTREMELY THERMOPHILIC ARCHAEBACTERIA (ARCHAEA)

被引：30

作者：

KAWAI, G

HASHIZUME, T

YASUDA, M

MIYAZAWA, T

MCCLOSKEY, JA

YOKOYAMA, S

机构：

[1] UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

[2] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

[3] UNIV UTAH,DEPT BIOCHEM,SALT LAKE CITY,UT 84112

[4] YOKOHAMA NATL UNIV,FAC ENGN,HODOGAYA KU,YOKOHAMA,KANAGAWA 240,JAPAN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1992年 / 11卷 / 2-4期

关键词：

D O I：

10.1080/07328319208021740

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The conformational characteristics of N4-acetyl-2'-O-methylcytidine (ac4Cm), which is one of the modified cytidines unique to the tRNA of extremely thermophilic archaebacteria, and related nucleosides, N4-acetylcytidine (ac4C), 2'-O-methylcytidine (Cm) and cytidine, were analyzed by proton nuclear magnetic resonance spectroscopy. Ribose methylation and N4-acylation were found to confer high conformational rigidity to the ribose moiety, suggesting that these post-transcriptional modifications play a role in structural stabilization of tRNA, which is of particular importance at high temperature.

引用

页码：759 / 771

页数：13

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