DETECTION OF ENTEROTOXINS AND TSST-1 SECRETED BY STAPHYLOCOCCUS-AUREUS ISOLATED FROM RUMINANT MASTITIS - COMPARISON OF ELISA AND IMMUNOBLOT

被引：37

作者：

ORDEN, JA ^{[1
]}

GOYACHE, J ^{[1
]}

HERNANDEZ, J ^{[1
]}

DOMENECH, A ^{[1
]}

SUAREZ, G ^{[1
]}

GOMEZLUCIA, E ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DIPARTIMENTO PATOL ANIM 1,E-28040 MADRID,SPAIN

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 72卷 / 06期

关键词：

D O I：

10.1111/j.1365-2672.1992.tb01863.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of staphylococcal enterotoxins (SE) and toxic shock syndrome toxin-1 (TSST-1) was studied in 81 strains of Staphylococcus au"us isolated from cases of mastitis in cattle, goats and sheep. SE and TSST-1 were detected by two techniques: ELISA double antibody sandwich, and an immunoblot technique combined with a semiautomated electrophoresis system. More Staph. aureus strains isolated from sheep produced enterotoxins than those from goats and cattle. SEC was the predominant type in all isolates from these animal species. The highest proportion of strains producing TSST-1 were obtained from sheep, twice as many as those from goats or cows. The two techniques gave similar results, as all the strains positive by immunoblot were also positive by ELISA, and only three were positive by ELISA but negative by immunoblot.

引用

页码：486 / 489

页数：4

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