ENZYMATIC-SYNTHESIS OF OCTYL-BETA-GLUCOSIDE IN OCTANOL AT CONTROLLED WATER ACTIVITY

被引：106

作者：

LJUNGER, G ^{[1
]}

ADLERCREUTZ, P ^{[1
]}

MATTIASSON, B ^{[1
]}

机构：

[1] LUND UNIV, CTR CHEM, DEPT BIOTECHNOL, S-22100 LUND, SWEDEN

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 09期

关键词：

BETA-GLUCOSIDASE; ALKYL GLYCOSIDE; WATER ACTIVITY; ORGANIC MEDIA;

D O I：

10.1016/0141-0229(94)90031-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of beta-glucosidase far synthesizing octyl-beta-glucoside was studied. Almond beta-glucosidase immobilized on XAD-4, glucose, and octanol were mixed at controlled water activity. A water activity of at least 0.67 was needed for synthesis of octyl-beta-glucoside, and the reaction rate increased with increasing water activity. The final yield decreased with increasing water activity. The best results were therefore obtained using a high initial water activity which was decreased during the course of the reaction. With the conditions chosen it was possible to reuse the enzyme several times.

引用

页码：751 / 755

页数：5

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