A FLOW-THROUGH MICROSAMPLING DEVICE APPLIED TO AN ION-EXCHANGE CHROMATOGRAPHY SYSTEM

被引：16

作者：

NILSSON, J

SZECSI, P

SCHAFERNIELSEN, C

机构：

[1] MALMO GEN HOSP,DEPT CLIN CHEM,S-21401 MALMO,SWEDEN

[2] GLOSTRUP CTY HOSP,DEPT CLIN CHEM,DK-2600 GLOSTRUP,DENMARK

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1993年 / 27卷 / 03期

关键词：

PIEZOELECTRIC DROP GENERATOR; FLOW-THROUGH LIQUID SAMPLER; FUSED ROCKET ELECTROPHORESIS;

D O I：

10.1016/0165-022X(93)90002-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A flow-through microsampling device that can be inserted in the column elution flow line of a chromatographic system has been developed. A sample drop (volume 2.2 nl) is ejected when a voltage pulse is applied to a piezo-electric transducer. The drops can be ejected with up to 70 Hz frequency, giving a sample flow range of 0-9 mul/min. Samples were collected on a moving agarose surface during elution of freshly prepared human serum. The precipitate pattern obtained after immunoelectrophoresis is similar to what would be obtained in a fused rocket electrophoresis of multiple samples from a fraction collector.

引用

页码：181 / 190

页数：10

共 11 条

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