INTERMEDIATES IN THE FOLDING OF THE MEMBRANE-PROTEIN BACTERIORHODOPSIN

被引：106

作者：

BOOTH, PJ

FLITSCH, SL

STERN, LJ

GREENHALGH, DA

KIM, PS

KHORANA, HG

机构：

[1] MIT,HOWARD HUGHES MED INST,WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA

[2] UNIV OXFORD,DYSON PERRINS LAB,OXFORD OX1 3QY,ENGLAND

[3] MIT,DEPT BIOL,CAMBRIDGE,MA

[4] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

NATURE STRUCTURAL BIOLOGY | 1995年 / 2卷 / 02期

关键词：

D O I：

10.1038/nsb0295-139

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Assembly of proteins within lipid bilayers is essential for the biogenesis and function of biological membranes. Little is known, however, about the underlying mechanism of assembly, and it is not clear whether it is possible to observe individual folding steps for integral membrane proteins either in vivo or in vitro. Fluorescence spectroscopy is used here to follow the time course of folding events for bacteriorhodopsin in mixed detergent/lipid micelles. Transient folding-intermediates are detected and binding of the retinal chromophore occurs at a late stage, when it kinds to an apoprotein intermediate.

引用

页码：139 / 143

页数：5

共 31 条

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