ENUMERATION OF NITRITE-OXIDIZING BACTERIA IN GRASSLAND SOILS USING A MOST PROBABLE NUMBER TECHNIQUE - EFFECT OF NITRITE CONCENTRATION AND SAMPLING PROCEDURE

被引:21
作者
BOTH, GJ [1 ]
GERARDS, S [1 ]
LAANBROEK, HJ [1 ]
机构
[1] INST ECOL RES,HETEREN,NETHERLANDS
关键词
NITROBACTER; INCUBATION MEDIUM; SPATIAL DISTRIBUTION;
D O I
10.1016/0378-1097(90)90680-O
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Numbers of nitrite-oxidizing bacteria were determined in grassland soils using a Most Probable Number technique. Two concentrations of nitrite were used in the incubation medium, i.e. 0.05 and 5.0 mM. The results of the enumerations were highly dependent on the nitrite concentration as well as on the grassland soil sampled. In one soil the highest numbers were counted with 5.0 mM, whereas in other soils highest numbers were obtained in media with 0.05 mM nitrite. The spatial distribution of nitrite-oxidizing bacteria was determined in two field plots. Variation between individual samples were low in one plot and high in the other. The implications of the observed differences for the sampling procedure in each sampling plot are discussed. In the waterlogged, oxygen-limited soils, numbers of nitrite-oxidizing bacteria were as high as in the drained soils. The chemolitho-autotrophic nature of these nitrifiers has been confirmed.
引用
收藏
页码:277 / 285
页数:9
相关论文
共 28 条
[1]  
Alexander M., 1982, Methods of soil analysis. Part 2. Chemical and microbiological properties, P815
[2]  
Alexander M., 1965, METHODS SOIL ANAL 2, V9, P1477, DOI 10.2134/agronmonogr9.2.c51
[3]   USE OF NITRIFIER ACTIVITY MEASUREMENTS TO ESTIMATE THE EFFICIENCY OF VIABLE NITRIFIER COUNTS IN SOILS AND SEDIMENTS [J].
BELSER, LW ;
MAYS, EL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (04) :945-948
[4]   POPULATION ECOLOGY OF NITRIFYING BACTERIA [J].
BELSER, LW .
ANNUAL REVIEW OF MICROBIOLOGY, 1979, 33 :309-333
[5]  
BERG P, 1986, THESIS SWEDISH U AGR
[7]   KINETICS OF NITRITE OXIDATION BY NITROBACTER WINOGRADSKYI [J].
BOON, B ;
LAUDELOUT, H .
BIOCHEMICAL JOURNAL, 1962, 85 (03) :440-&
[8]  
BOTH GJ, 1990, UNPUB MOST PROBABLE
[9]   AUTOTROPHIC NITRIFICATION IN A FERTILIZED ACID HEATH SOIL [J].
DEBOER, W ;
DUYTS, H ;
LAANBROEK, HJ .
SOIL BIOLOGY & BIOCHEMISTRY, 1988, 20 (06) :845-850
[10]  
Focht D.D., 1977, ADV MICROB ECOL, P135, DOI DOI 10.1007/978-1-4615-8219-9_4