DETECTION OF PHOTOREPAIR OF UV-INDUCED THYMINE DIMERS IN HUMAN EPIDERMIS BY IMMUNOFLUORESCENCE MICROSCOPY

To investigate the effect of visible light on the level of UV-induced thymine dimers in human epidermal cells in vivo, we exposed volunteers to UV-B alone, or to a serial combination of UV-B and visible light. Dimers were assayed in skin sections by immunofluorescence microscopy with a monoclonal antibody against the cyclobutyl thymine dimer. The dimer-specific fluorescence from epidermal cell nuclei, identified by counterstaining with propidium iodide, was quantified through computer-mediated image processing and analysis. After a single UV exposure (2-3 MED), significant dimer-specific fluorescence was measured, but no difference could be detected between skin kept in the dark after UV-irradiation and that exposed to visible light. In three other experiments, the UV dose was split into 3 parts (1 MED each), given at 2.5-h intervals. Half of the skin area was exposed to visible light following each dose fraction. After the second and third dose fractions, skin areas treated with visible light clearly showed lower levels of dimers (i.e., about 40% reduced) than skin kept in the dark. The results provide evidence that photorepair of dimers does occur in human skin, but not immediately after a first UV exposure of naive skin.