DIRECT SEQUENCING OF LARGE FLAVIVIRUS PCR PRODUCTS FOR ANALYSIS OF GENOME VARIATION AND MOLECULAR EPIDEMIOLOGIC INVESTIGATIONS

被引：62

作者：

LEWIS, JG

CHANG, GJ

LANCIOTTI, RS

TRENT, DW

机构：

[1] Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control, Fort Collins, CO

来源：

JOURNAL OF VIROLOGICAL METHODS | 1992年 / 38卷 / 01期

关键词：

DENGUE; POLYMERASE CHAIN REACTION (PCR); SEQUENCING;

D O I：

10.1016/0166-0934(92)90165-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction (PCR) was used to amplify viral cDNAs from selected regions of dengue genomic RNA by using appropriate 'consensus' primers. DNA amplicons containing the structural genes from all 4 dengue serotypes were prepared and directly sequenced using dengue-virus-specific primers. This method can characterize reliably flavivirus field isolates at the molecular level without extensive virus propagation and molecular cloning, and will be a valuable tool for molecular epidemiological studies.

引用

页码：11 / 23

页数：13

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