ORIGIN OF SINGLE PRONUCLEATED HUMAN ZYGOTES

被引：31

作者：

MUNNE, S

TANG, YX

GRIFO, J

COHEN, J

机构：

[1] The Gamete and Embryo Research Laboratory, Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York, 10021

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 04期

关键词：

IN-VITRO FERTILIZATION; POLYMERASE CHAIN REACTION; IN-SITU HYBRIDIZATION; SINGLE PRONUCLEATED ZYGOTES; PREIMPLANTATION GENETICS;

D O I：

10.1007/BF01204942

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Diploidy in embryos developing from single pronucleated zygotes can occur following parthenogenetic activation or by asynchronous inflation of pronuclei following fertilization. To distinguish between these two mechanisms, sexing was performed. Results: The presence of a Y chromosome in the embryo was considered proof that fertilization had occurred. Twenty-one dividing embryos originating from single pronucleated zygotes were analyzed using the polymerase chain reaction or fluorescence in situ hybridization. In total, 43% (9121) of the embryos showed Y chromosomes. Conclusion: It can be extrapolated that more than 80% of them originated from fertilized eggs.

引用

页码：276 / 279

页数：4

共 17 条

[1]

Abramczuk J.W., Lopata A., Resistance of human follicular oocytes to parthenogenesic activation: DNA distribution and content in oocytes maintained in vitro, Hum Reprod, 5, pp. 578-581, (1990)

[2]

Winston N., Johnson M., Pickering S., Braude P., Parthenogenetic activation and development of fresh and aged human oocytes, Fertil Steril, 56, pp. 904-912, (1991)

[3]

Plachot M., Chromosome analysis of oocytes and embryos, Preimplantation Genetics, pp. 103-112, (1991)

[4]

Staessen C., Janssenwillen C., Devroey P., Van Steirteghem A.C., Cytogenetic and morphological observations of single pronucleated human oocytes after in-vitro fertilization, Hum Reprod, 8, pp. 221-223, (1993)

[5]

Kaufman M., Early Mammalian Development: Parthenogenetic Studies, (1983)

[6]

Xu K.P., Greve T., A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes, J Reprod Fertil, 82, pp. 127-134, (1988)

[7]

Munne S., Weier H.U.G., Stein J., Grifo J., Cohen J., A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres, Journal of Assisted Reproduction and Genetics, 10, pp. 82-90, (1993)

[8]

Handyside A.H., Kontogianni E.H., Hardy K., Winston R.M.L., Pregnancies from biopsied human pre-implantation embryos sexed by Y-specific DNA amplification, Nature, 344, pp. 768-770, (1990)

[9]

Grifo J.A., Tang Y.X., Cohen J., Gilbert F., Sanyal M.K., Rosenwaks Z., Ongoing pregnancy in a hemophilia carrier by embryo biopsy and simultaneous amplification of X and Y chromosome specific DNA from single blastomeres, JAMA, 6, pp. 727-729, (1992)

[10]

Griffin D.K., Wilton L.J., Handyside A.H., Winston R.M.L., Delhanty J.D.A., Dual fluorescent in situ hybridization for simultaneous detection of X and Y chromosome-specific probes for the sexing of human preimplantation embryonic nuclei, Hum Genet, 89, pp. 18-22, (1992)

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