FATTY-ACID AND POLAR LIPID-COMPOSITION OF THE GENUS AMYCOLATOPSIS - APPLICATION OF FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY TO STRUCTURE-ANALYSIS OF UNDERIVATIZED PHOSPHOLIPIDS

被引：69

作者：

YASSIN, AF ^{[1
]}

HAGGENEI, B ^{[1
]}

BUDZIKIEWICZ, H ^{[1
]}

SCHAAL, KP ^{[1
]}

机构：

[1] UNIV COLOGNE, INST ORGAN CHEM, W-5000 COLOGNE 41, GERMANY

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1993年 / 43卷 / 03期

关键词：

D O I：

10.1099/00207713-43-3-414

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Phospholipid patterns of 15 representative strains of the genus Amycolatopsis were recorded by two-dimensional thin-layer chromatography. The structure analysis of the isolated phospholipids was verified by fast atom bombardment-mass spectroscopy. The positive- and negative-ion spectra of the partially purified phospholipid fractions qualitatively reflect their distinctive composition. All strains contained diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidylinositol. Two different types of phosphatidylethanolamine and phosphatidylmethylethanolamine were detected, viz., compounds with or without hydroxy fatty acids. These phospholipid patterns underline the integrity of the genus. Fast atom bombardment-mass spectrometry analysis of phospholipid patterns may serve as an aid for differentiation of bacterial species.

引用

页码：414 / 420

页数：7

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