PREBIOTIC SYNTHESIS - PHOSPHORYLATION IN AQUEOUS SOLUTION

Formation of uridine-5′-phosphate from uridine and inorganic phosphate in aqueous solution is effected by the following condensing agents: cyanogen, cyanoformamide, cyanate, cyanamide, thioformate, ethylisocyanide, and a water-soluble carbodiimide. The yields are always small, even when a large excess of condensing agent is used. The cycligation of uridine-3′- (2′)-phosphate occurs under the same conditions but in much greater yield.