COMBINED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC RADIOIMMUNOASSAY METHODS FOR THE BIOANALYSIS OF PEPTIDES

被引：3

作者：

VENN, RF

机构：

[1] Pain Research Institute, Liverpool, L9 1AE, Rice Lane

来源：

JOURNAL OF CONTROLLED RELEASE | 1990年 / 13卷 / 2-3期

关键词：

bioanalysis of peptides; HPLC; neuropeptides; analytical methodology; RIA;

D O I：

10.1016/0168-3659(90)90002-B

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Some of the attributes of and criteria for establishing reliable assays for the bioanalysis of peptides are discussed, together with descriptions of some of the difficulties encountered in determining the concentrations of biologically active peptides. The use of a method combining the sensitivity and specificity of radioimmunoassay with the extra specificity of high-pressure liquid chromatography is described. This combination overcomes many of the problems such as cross-reactivity of related peptides in such assays, and allows many peptides to be assayed from one sample. © 1990.

引用

页码：113 / 120

页数：8