CONCURRENT C18-SOLID PHASE EXTRACTION OF PLATELET-ACTIVATING-FACTOR (PAF) AND ARACHIDONIC-ACID METABOLITES

被引：6

作者：

BIOQUE, G ^{[1
]}

TOST, D ^{[1
]}

CLOSA, D ^{[1
]}

ROSELLOCATAFAU, J ^{[1
]}

RAMIS, I ^{[1
]}

CABRER, F ^{[1
]}

CARGANICO, G ^{[1
]}

GELPI, E ^{[1
]}

机构：

[1] LABS MENARINI, BARCELONA, SPAIN

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 08期

关键词：

D O I：

10.1080/10826079208018285

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Concurrent solid phase extraction of arachidonic acid metabolites and platelet activating factor in human vein umbilical endotelial incubates has been achieved on commercial C18 reversed phase cartridges. Control of sample pH, elution solvents and C18 adsorbent material are important for selective recoveries. Samples were acidified at pH = 4.0 and processed through the C18 cartridges which were washed with 10 ml of water/methanol 9/1, 1 0 ml of water at pH = 4.0 and 20 ml of petroleum ether. Arachidonic acid metabolites and platelet activating factor adsorbed on the solid phase material were sequentially separated by elution with 4.5 ml of methyl formate and 4.5 ml of methanol, respectively. Recoveries thus obtained were 65.0 +/- 2.5%, 89.1 +/- 4.6%, 91.1 +/- 7.5%, 94.8 +/- 1.6 % for PAF, 6-keto PGF1-alpha, 15-HETE and PGE2 respectively. The method was also compared with the classical Bligh & Dyer liquid-liquid extraction procedure.

引用

页码：1249 / 1258

页数：10

共 17 条

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[2]

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