HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BIOTIN IN PHARMACEUTICAL PREPARATIONS BY POSTCOLUMN FLUORESCENCE REACTION WITH THIAMINE REAGENT

被引：8

作者：

YOKOYAMA, T ^{[1
]}

KINOSHITA, T ^{[1
]}

机构：

[1] KITASATO UNIV, SCH PHARMACEUT SCI, TOKYO 108, JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 542卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)88775-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method for the determination of biotin was devised utilizing a post-column reaction in which the amide bond of biotin was chlorinated and allowed to react with thiamine to give fluorescent thiochrome. A linear relationship was observed between the peak height of biotin in the range 20 ng-3-mu-g per injection. The limit of detection was 10 ng per injection. The relative standard deviations for 40 and 200 ng per injection of biotin were 3.1 and 1.2% (n = 10), respectively. The method could be applied directly to pharmaceutical preparations without any sample pretreatment for the elimination of interfering materials as is required in the conventional methods.

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页码：365 / 372

页数：8

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