SEQUENCE SPECIFICITY OF CYCLOBUTANE PYRIMIDINE DIMERS IN DNA TREATED WITH SOLAR (ULTRAVIOLET-B) RADIATION

被引：155

作者：

MITCHELL, DL ^{[1
]}

JEN, J ^{[1
]}

CLEAVER, JE ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,RADIOBIOL & ENVIRONM HLTH LAB,SAN FRANCISCO,CA 94143

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 02期

关键词：

D O I：

10.1093/nar/20.2.225

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cyclobutane pyrimidine dimers were quantified at the sequence level after irradiation with solar ultraviolet (UVB) and nonsolar ultraviolet (UVC) light sources. The yield of photoproducts at specific sites was dependent on the nucleotide composition in and around the potential lesion as well as on the wavelength of ultraviolet light used to induce the damage. Induction was greater in the presence of 5' flanking pyrimidines than purines; 5' guanine inhibited induction more than adenine. UVB irradiation increased the induction of cyclobutane dimers containing cytosine relative to thymine homodimers. At the single UVC and UVB fluences used, the ratio of thymine homodimers (T [] T) to dimers containing cytosine (C [] T, T [] C, C [] C) was greater after UVC compared to UVB irradiation.

引用

页码：225 / 229

页数：5

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