CHARACTERIZATION OF PROTEASES PRODUCED BY NEWLY ISOLATED AND IDENTIFIED PROTEOLYTIC MICROORGANISMS FROM FERMENTED FISH (BUDU)

被引:11
作者
CHOORIT, W [1 ]
PRASERTSAN, P [1 ]
机构
[1] PRINCE SONGKLA UNIV,FAC NAT RESOURCES,DEPT AGROIND,HAT YAI 90110,THAILAND
关键词
BACILLUS; BUDU; FERMENTATION; FISH; PROTEASES;
D O I
10.1007/BF01201880
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Eight different strains of Bacillus were isolated from fermented fish (Budu) and their proteolytic enzyme activities were determined after 18 h cultivation at room temperature (35-degrees-C). Four isolates possessed high protease activities. Optimum pH for these enzymes was between 7.0 and 8.0 and the optimal temperature was 55-degrees-C. The proteases retained 40% of their original activity after 20 min at 55-degrees-C but lost all activity at 65-degrees-C. Three of the four isolates were identified as Bacillus subtilis, the fourth as Bacillus licheniformis.
引用
收藏
页码:284 / 286
页数:3
相关论文
共 9 条
[1]  
Buchanan R., Bergey's Manual of Determinative Bacteriology, (1974)
[2]  
Crisan E.V., Sands A., Microflora of four fermented fish sauces, Applied Microbiology, 29, pp. 106-108, (1975)
[3]  
Durham D.R., Stewart D.B., Stellwag E.J., Novel alkaline- and heat-stable serine proteases from alkalophilic Bacillus sp. strain GX 6638, Journal of Bacteriology, 169, pp. 2762-2768, (1987)
[4]  
Fujii M., Takagi M., Imanaka T., Aiba S., Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis, Journal of Bacteriology, 154, pp. 831-837, (1983)
[5]  
Hagihara B., Matsubara H., Nakai M., Okunuki K., Crystalline bacterial proteinase. I. Preparation of crystalline proteinase of Bac. subtilis, Journal of Biochemistry (Tokyo), 45, pp. 185-194, (1958)
[6]  
Ho C.C., Toh S.E., Ajam N., Cheah K.P., Isolation and characterization of halophilic yeast and bacteria involved in soy sauce fermentation in Malaysia, Food Technology (Australia), 36, pp. 227-230, (1984)
[7]  
Priest F.G., Extracellular enzyme synthesis in the genus Bacillus, Bacteriological Reviews, 41, pp. 711-753, (1977)
[8]  
Uehara H., Yamana K., Maruo B., Thermosensitive, extracellular neutral proteinase in Bacillus subtilis: isolation, characterization and genetics, Journal of Bacteriology, 139, pp. 583-590, (1973)
[9]  
Zuberi R., Shahad S.I., Qadri R.B., Bacterial flora of fermented fish sauce prepared from sardines in Pakistan, Tropical Science, 28, pp. 239-246, (1988)