CHARACTERIZATION OF A LINE-1 CDNA THAT ORIGINATED FROM RNA PRESENT IN RIBONUCLEOPROTEIN-PARTICLES - IMPLICATIONS FOR THE STRUCTURE OF AN ACTIVE-MOUSE LINE-1

被引：22

作者：

MARTIN, SL

机构：

[1] Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver

来源：

GENE | 1995年 / 153卷 / 02期

关键词：

GENE EXPRESSION; REPETITIVE DNA; RETROTRANSPOSON;

D O I：

10.1016/0378-1119(94)00785-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Full-length, sense-strand, long interspersed element-1 (LINE-1 or L1) RNA is found as an RNA-protein complex in mouse embryonal carcinoma cells, Since this complex is a likely intermediate in LINE-1 transposition, its RNA may be enriched for the functional, or active, subset of mouse L1 sequences. For this reason, a cDNA library was constructed from RNA prepared from these ribonucleoprotein particles. The isolation and complete DNA sequence of one clone that is a strong candidate to be a functional version of mouse L1 is reported here. The structure of this element suggests a revision of the predicted sequence of an active mouse L1 and provides a tag that can be used to isolate its locus in the genome.

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页码：261 / 266

页数：6

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