DEVELOPMENTAL EXPRESSION OF ACTIVIN INHIBIN BETA(A)-SUBUNITS, BETA(B)-SUBUNITS AND ALPHA-SUBUNITS, AND ACTIVIN RECEPTOR-IIB GENES IN PREIMPLANTATION MOUSE EMBRYOS

被引:41
作者
LU, RZ [1 ]
MATSUYAMA, S [1 ]
NISHIHARA, M [1 ]
TAKAHASHI, M [1 ]
机构
[1] UNIV TOKYO,DEPT VET PHYSIOL,1-1-1 YAYOI,BUNKYO KU,TOKYO 113,JAPAN
关键词
D O I
10.1095/biolreprod49.6.1163
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We demonstrated previously that activin A released the two-cell block of mouse embryos cultured in vitro and stimulated early embryonic development. We then confirmed immunohistochemically the presence of the beta(A), beta(B), and alpha subunits in early embryos together with the oviductal epithelium facing those embryos. The results of in situ hybridization and reverse-transcription polymerase chain reaction here show the presence of mRNA transcripts for activin/inhibin beta(A), beta(B) subunits in the ovary, oviduct, unfertilized egg, and embryo at the early preimplantation stage. However, the mRNA of the inhibin alpha subunit was not expressed in any of these. Considering our previous demonstration of the immunoreactive B(A), and beta(B) subunits of activin/inhibin polypeptides in the cytoplasm of 1- and 2-cell embryos, we suggest that activins appearing in the oviduct and in embryos are not only transferred from the follicular fluids, but produced by the oviduct, oocytes, and embryos themselves. Since the mRNA of the inhibin alpha subunit was absent at those stages, the beta(A) and beta(B) subunits may not exist as the inhibin molecule. The mRNA of activin receptor-IIB was detected in the ovary, in embryos at the 1-cell and the 8-cell/morula stages, and also in the unfertilized egg, although to a lesser extent, but not in the oviduct or in the 2-cell-stage embryo either in vivo or cultured in vitro. These results suggest that activin is physiologically involved in the process of early development of mouse embryos.
引用
收藏
页码:1163 / 1169
页数:7
相关论文
共 46 条
[1]  
ALBANO RM, 1993, DEVELOPMENT, V117, P711
[2]   NOVEL ACTIVIN RECEPTORS - DISTINCT GENES AND ALTERNATIVE MESSENGER-RNA SPLICING GENERATE A REPERTOIRE OF SERINE THREONINE KINASE RECEPTORS [J].
ATTISANO, L ;
WRANA, JL ;
CHEIFETZ, S ;
MASSAGUE, J .
CELL, 1992, 68 (01) :97-108
[3]   POLYADENYLATED RNA OF MOUSE OVA AND LOSS OF MATERNAL RNA IN EARLY DEVELOPMENT [J].
BACHVAROVA, R ;
DELEON, V .
DEVELOPMENTAL BIOLOGY, 1980, 74 (01) :1-8
[4]   INHIBITION OF SOMATOTROPH GROWTH AND GROWTH-HORMONE BIOSYNTHESIS BY ACTIVIN INVITRO [J].
BILLESTRUP, N ;
GONZALEZMANCHON, C ;
POTTER, E ;
VALE, W .
MOLECULAR ENDOCRINOLOGY, 1990, 4 (02) :356-362
[5]  
BRONXMEYER H, 1988, P NATL ACAD SCI USA, V85, P9052
[6]   CHARACTERIZATION OF ACTIVIN-A BINDING-SITES ON THE HUMAN-LEUKEMIA CELL-LINE K562 [J].
CAMPEN, CA ;
VALE, W .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (02) :844-849
[7]   ACTIVIN-A BINDING AND BIOCHEMICAL EFFECTS IN OSTEOBLAST-ENRICHED CULTURES FROM FETAL-RAT PARIETAL BONE [J].
CENTRELLA, M ;
MCCARTHY, TL ;
CANALIS, E .
MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (01) :250-258
[8]   RNA-SYNTHESIS AND CYTOPLASMIC POLYADENYLATION IN THE ONE-CELL MOUSE EMBRYO [J].
CLEGG, KB ;
PIKO, L .
NATURE, 1982, 295 (5847) :342-345
[9]   ALPHA-INHIBIN GENE-EXPRESSION OCCURS IN THE OVINE ADRENAL-CORTEX, AND IS REGULATED BY ADRENOCORTICOTROPIN [J].
CRAWFORD, RJ ;
HAMMOND, VE ;
EVANS, BA ;
COGHLAN, JP ;
HARALAMBIDIS, J ;
HUDSON, B ;
PENSCHOW, JD ;
RICHARDS, RI ;
TREGEAR, GW .
MOLECULAR ENDOCRINOLOGY, 1987, 1 (10) :699-706
[10]   INHIBIN [J].
DEJONG, FH .
PHYSIOLOGICAL REVIEWS, 1988, 68 (02) :555-607