ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .14. USE OF DRAINAGE SYPHONS IN A FAST-ANALYZER CUVET-ROTOR

A cuvet-rotor, designated G-IIB, has been developed that contains small-bore syphons attached to each of the fifteen cuvets. Drainage of reactants from the transfer disc was found to be approximately 90% complete in 1 second and 99% complete after 2.5 seconds during acceleration to 1000 rpm. Mixing of protein-biuret reagent solution and 30% sucrose occurred in 1 second when a vacuum was used to pull air back through the syphons and cuvets. Drainage of the cuvets through the syphons was almost quantitative (>99%) when air pressure and one cycle from 400 rpm to 1000 rpm and back to 400 rpm was used. The interval between successive sets of analyses may therefore be 1 minute or less. A method of heat cycling to make the Telflon cuvet-spacer conform to the glass windows is presented. © 1969.