METABOLISM OF PURINES AND THEIR DERIVATIVES IN SEEDLINGS OF PISUM-SATIVUM

被引:19
作者
SILVER, AV
GILMORE, V
机构
[1] Department of Biology, University of Salford, Salford
关键词
D O I
10.1016/S0031-9422(00)88142-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pattern of metabolism of soluble purines infiltrated into pea seedlings has been investigated. Nucleotides were converted to the corresponding nucleosides and free bases. Nucleosides were converted to the corresponding free bases. Deamination of the adenine series of compounds to the hypoxanthine series occurred at nucleotide level. Oxidation of the hypoxanthine series of compounds to the xanthine series took place at free base level, providing strong evidence for the presence of xanthine oxidase (xanthine: O2 oxido-reductase, EC 1.2.3.2) in these seedlings. Sub-cellular fractions from pea seedlings were prepared and tested for activity against nucleotide substrates. Hydrolytic degradation of these nucleotides by the soluble cell fraction was observed, but all particulate fractions were inactive. The possible intracellular compartmentalization of endogenous purine derivatives is discussed. © 1969.
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页码:2295 / &
相关论文
共 11 条
[1]   FORMATION OF ALLANTOIN AND ALLANTOIC ACID FROM ADENINE IN LEAVES OF ACER-SACCHARINUM L [J].
BARNES, RL .
NATURE, 1959, 184 (4703) :1944-1944
[2]  
BARNES ROBERT L., 1961, BOT GAZ, V123, P141, DOI 10.1086/336140
[3]   STRUCTURE OF A PYRIMIDINE AMINO ACID FROM PEA SEEDLINGS [J].
BROWN, EG ;
MANGAT, BS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 177 (03) :427-+
[4]   PORINE AND PYRIMIOINE DERIVATIVES IN MATURE PEA SEEDS [J].
BROWN, EG .
BIOCHEMICAL JOURNAL, 1963, 88 (03) :498-&
[5]   ACID-SOLUBLE NUCLEOTIDES OF MATURE PEA SEEDS [J].
BROWN, EG .
BIOCHEMICAL JOURNAL, 1962, 85 (03) :633-&
[6]   NATURAL OCCURRENCE OF A URACIL 5-PEPTIDE AND ITS METABOLIC RELATIONSHIP TO GUANOSINE 5'-MONOPHOSPHATE [J].
BROWN, EG ;
SILVER, AV .
BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 119 (01) :1-&
[7]  
Fosse R, 1932, CR HEBD ACAD SCI, V195, P1198
[8]  
MAGASANIK B, 1960, J BIOL CHEM, V235, P2672
[9]   INTERCONVERSION OF PURINES IN THE BIOSYNTHESIS OF NUCLEIC ACID ADENINE AND GUANINE AND OF HISTIDINE [J].
MAGASANIK, B ;
MOYED, HS ;
KARIBIAN, D .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1956, 78 (07) :1510-1511
[10]  
MAGER J, 1958, FED PROC, V17, P267