MEASUREMENT BY QUANTIMET 720 OF THE PROPORTION OF ACTIVELY GROWING-CELLS IN TISSUE-CULTURES OF HUMAN LYMPHOCYTES

被引:21
作者
BROWN, RA
MCWALTER, R
SLIDDERS, W
GIBBS, J
SWANSON, J
机构
[1] UNIV DUNDEE,DEPT PATHOL,DUNDEE DD1 4HN,SCOTLAND
[2] NINEWELLS HOSP & MED SCH,DUNDEE DD1 9SY,SCOTLAND
来源
JOURNAL OF MICROSCOPY-OXFORD | 1979年 / 115卷 / JAN期
关键词
D O I
10.1111/j.1365-2818.1979.tb00151.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
The size‐distribution of normal human lymphocytes growing in tissue culture was measured with a Quantimet 720 image‐analysing computer. The proportion of cells undergoing volumetric growth after phytohaemagglutinin (PHA) stimulation was derived from a simple mathematical model describing the growth patterns of the cells up to the first mitotic division. The approach may have general application in biology as a method for measuring the proportion of growing cells by microscopy. 1979 Blackwell Science Ltd
引用
收藏
页码:51 / 63
页数:13
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