PURIFICATION AND CHARACTERIZATION OF 3,4-DIHYDROXYPHENYLALANINE DECARBOXYLASE FROM PIG KIDNEY

被引：35

作者：

BORRIVOLTATTORNI, C ^{[1
]}

MINELLI, A ^{[1
]}

VECCHINI, P ^{[1
]}

FIORI, A ^{[1
]}

TURANO, C ^{[1
]}

机构：

[1] UNIV ROME,FAC PHARM,INST BIOL CHEM,I-00100 ROME,ITALY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 93卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb12809.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for 3,4‐dihydroxyphenylalanine decarboxylase from pig kidney purification is described in detail. The preparation has no detectable impurity on elecrophoresis and on ultracentrifugation and has a coenzyme content and a specific activity comparable with the same enzyme purified by other authors. However two significant differences are observed: a different stimulation of activity by added pyridoxal 5′‐phosphate and a nearly complete decarboxylation of L‐3,4‐dihydroxyphenylalanine in absence of added coenzyme. Absorption, fluorescence and circular dichroism properties of the coenzyme‐apoenzyme interaction are also described. The results are consistent with the existence of at least four coenzyme‐apoenzyme complexes, three of them active. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：181 / 188

页数：8

共 21 条

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