C-13 NMR-STUDIES ON FLUORESCENT-PROBES - C-13 CHEMICAL-SHIFTS AND LONGITUDINAL RELAXATION-TIMES OF N-HYDROXY-FATTY (N=2,6,9,12) ACIDS AND N-(9-ANTHROYLOXY)-STEARIC (N=6,12) ACIDS

被引:16
作者
JOHNS, SR [1 ]
WILLING, RI [1 ]
THULBORN, KR [1 ]
SAWYER, WH [1 ]
机构
[1] UNIV MELBOURNE,RUSSELL GRIMWADE SCH BIOCHEM,PARKVILLE 3052,VICTORIA,AUSTRALIA
基金
澳大利亚研究理事会;
关键词
D O I
10.1016/0009-3084(79)90091-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
13C NMR has been used to confirm the structure of two fluorescent probes, n-(9-anthroyloxy)-stearic acids (n=6,12), and the series of n-hydroxy-fatty acids (n=2,6,9,12) from which the set of fluorescent fatty acids may be synthesised. 13C longitudinal relaxation times and correlation times of the individual carbon atoms in 12-hydroxy- and 6- and 12-(9-anthroyloxy)-stearic acids show differences in motional properties between these derivatives and the parent stearic acid in chloroform(d) solution. The correlation times of the substituted carbons in 6-, 9-, and 12-hydroxy-stearic acids are longer than the corresponding carbons in stearic acid. The change in correlation times at the substituted carbons reflects the increase in motion along the acyl chain. Attachment of the bulky anthracene ring causes greater restriction of motion at the substituted carbon atom but the gradient of motion along the chain is preserved. These results are discussed in terms of the types of motion which lead to fluorescence depolarization when the fluorescent fatty acids are used as fluidity probes in biomembranes. © 1979.
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页码:11 / 16
页数:6
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