DETECTION OF INDUCIBLE PROSTAGLANDIN-H SYNTHASE-2 IN CELLS IN THE EXUDATE OF RAT CARRAGEENAN-INDUCED PLEURISY

被引：39

作者：

HARADA, Y ^{[1
]}

HATANAKA, K ^{[1
]}

SAITO, M ^{[1
]}

MAJIMA, M ^{[1
]}

OGINO, M ^{[1
]}

KAWAMURA, M ^{[1
]}

OHNO, T ^{[1
]}

YANG, QS ^{[1
]}

KATORI, M ^{[1
]}

YAMAMOTO, S ^{[1
]}

机构：

[1] UNIV TOKUSHIMA,SCH MED,DEPT BIOCHEM,TOKUSHIMA 770,JAPAN

来源：

BIOMEDICAL RESEARCH-TOKYO | 1994年 / 15卷 / 02期

关键词：

D O I：

10.2220/biomedres.15.127

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Pleurisy was induced in rats by intrapleural injection of 0.2 ml of 2% lambda-carrageenin. Prostaglandin H synthase (PGHS)-2 was detected using immunoblot analysis in cells of the pleural exudate 5 h after carrageenin injection, and waned at 19 h. PGHS-1 was also detected, but was maintained at similar levels in cells before and after carrageenin injection. The level of PGHS-2 was markedly suppressed by pretreatment of rats with anti-inflammatory dose of dexamethasone (3 mg/kg, i.p.) 2 h before carrageenin injection. Furthermore, only PGHS-1, but not PGHS-2, was detected in the microsomal fraction of the lung, stomach and kidney of pleurisy-affected and non-affected normal rats. These results suggest the involvement of PGHS-2 in the prostanoid formation in the inflammatory site.

引用

页码：127 / 130

页数：4

共 14 条

[1] DeWitt D L, 1993, Am J Med, V95, p40S, DOI 10.1016/0002-9343(93)90396-7
[2] PROSTAGLANDIN ENDOPEROXIDE SYNTHASE - REGULATION OF ENZYME EXPRESSION
DEWITT, DL
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1083 (02) : 121 - 134
[3] NEW ASPECTS OF MODE OF ACTION OF NONSTEROID ANTIINFLAMMATORY DRUGS
FERREIRA, SH
VANE, JR
[J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1974, 14 : 57 - 73
[4] CHANGES IN THE LEVELS OF PROSTAGLANDINS AND THROMBOXANE AND THEIR ROLES IN THE ACCUMULATION OF EXUDATE IN RAT CARRAGEENIN-INDUCED PLEURISY - A PROFILE ANALYSIS USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY
HARADA, Y
TANAKA, K
UCHIDA, Y
UENO, A
OHISHI, S
YAMASHITA, K
ISHIBASHI, M
MIYAZAKI, H
KATORI, M
[J]. PROSTAGLANDINS, 1982, 23 (06): : 881 - 895
[5] HATSUYA S, 1980, KITASATO MED, V10, P528
[6] HUMAN CYCLOOXYGENASE-2 CDNA
HLA, T
NEILSON, K
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (16) : 7384 - 7388
[7] ISHIMURA K, 1993, HISTOCHEMISTRY, V99, P485
[8] KUJUBU DA, 1992, J BIOL CHEM, V267, P7991
[9] MEADE EA, 1993, J BIOL CHEM, V268, P6610
[10] SELECTIVITY OF NONSTEROIDAL ANTIINFLAMMATORY DRUGS AS INHIBITORS OF CONSTITUTIVE AND INDUCIBLE CYCLOOXYGENASE
MITCHELL, JA
AKARASEREENONT, P
THIEMERMANN, C
FLOWER, RJ
VANE, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (24) : 11693 - 11697

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