USE OF EXONUCLEASE-III FOR PREPARING SINGLE STRANDED DNA FOR USE AS A TEMPLATE IN THE CHAIN TERMINATOR SEQUENCING METHOD

Exonuclease III, which degrades DNA 3′ →5′ and is specific for duplex DNA, can be used to prepare single stranded DNA from linear duplexes. This is shown to be suitable as a template for use with the chain terminator DNA sequencing method of Sanger et al. [1]. Strategies are discussed for preparing single stranded DNA templates by this method and, in particular, its application to the sequence analysis of DNA cloned in plasmid vectors. © 1979 Information Retrieval Limited.