METABOLISM OF ILLUDIN-S, A TOXIC PRINCIPLE OF LAMPTEROMYCES-JAPONICUS, BY RAT-LIVER .1. ISOLATION AND IDENTIFICATION OF CYCLOPROPANE RING-CLEAVAGE METABOLITES

1. Illudin S, a toxic principle of the basidiomycete Lampteromyces japonicus, was incubated with rat liver 9000g supernatant and its metabolites studied. 2. Two metabolites, M1 and M2, were isolated and identified as cyclopropane ring-cleavage compounds by n.m.r., i.r. and mass spectral analyses. Moreover, M2 contained a chlorine atom. 3. On the basis of detailed analyses of the 2D n.m.r. spectra and differential nuclear Overhauser effect experiments, the previous assignments of the cyclopropane carbons of illudin S were revised. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.