THE EUROTRANSPLANT HLA-DRB OLIGONUCLEOTIDE TYPING SET

被引：30

作者：

GIPHART, MJ ^{[1
]}

VERDUIJN, W ^{[1
]}

机构：

[1] UNIV HOSP LEIDEN,EUROTRANSPLANT FDN,2300 RC LEIDEN,NETHERLANDS

来源：

EUROPEAN JOURNAL OF IMMUNOGENETICS | 1991年 / 18卷 / 1-2期

关键词：

D O I：

10.1111/j.1744-313X.1991.tb00006.x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Several sequence-specific oligonucleotides (SSO) were designed based upon available sequences of HLA-DRB1 genes which are associated with HLA-DR serotypes. Targets for these SSO were polymerase chain reaction (PCR) products using primers specific for the second exon of DRB genes. In view of practicality, a hybridization system was developed which was independent of temperature or composition of the hybridization buffers, hence uniformly applicable to all SSO, yet allowing discrimination based upon one nucleotide mismatch only. The SSO typing results were compared with HLA serological typing and a high correlation was observed (99%).

引用

页码：57 / 68

页数：12

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