A SIMPLE THEORETICAL TREATMENT OF A COMPETITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) AND ITS APPLICATION TO THE DETECTION OF HUMAN BLOOD-GROUP ANTIGENS

被引:21
作者
CHAPMAN, V
FLETCHER, SM
JONES, MN
机构
[1] UNIV MANCHESTER,SCH BIOL SCI,DEPT BIOCHEM & MOLEC BIOL,BIOMOLEC ORG & MEMBRANE TECHNOL GRP,MANCHESTER M13 9PT,LANCS,ENGLAND
[2] UNIV MANCHESTER,SCH BIOL SCI,DEPT BIOCHEM & MOLEC BIOL,MANCHESTER M13 9PT,LANCS,ENGLAND
关键词
Competitive assay; ELISA; Human blood group antigen; Ovarian cyst glycoprotein blood group activity; Theoretical model;
D O I
10.1016/0022-1759(90)90237-P
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A theory has been developed to explain the behaviour of a competitive enzyme-linked immunosorbent assay (ELISA) in which an immobilized antigen competes with a liquid-phase antigen for a limiting amount of antibody. The binding of antibody to antigen on a solid surface (microtitre well) is described in terms of Langmuirian adsorption with a binding constant k. Two equations are presented to describe the behaviour of the ELISA signal as a function of competing antigen concentration; an exact equation and an approximate equation which can be used when the surface coverage of the immobilized antigen is not known. It is shown how curves of ELISA signal vs. competing antigen concentration depend on K k[antibody]. The theory has been tested using several immobilized blood group A antigens competing with ovarian cyst fluid A substance and found to adequately describe these competitive ELISAs which have a detection limit of approximately 1 ng of blood group antigen. © 1990.
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页码:91 / 98
页数:8
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