M-TUBERCULOSIS-COMPLEX SPECIFIC T-CELL STIMULATION AND DTH REACTIONS INDUCED WITH A PEPTIDE FROM THE 38-KDA PROTEIN

被引：31

作者：

VORDERMEIER, HM ^{[1
]}

HARRIS, DP ^{[1
]}

MEHROTRA, PK ^{[1
]}

ROMAN, E ^{[1
]}

ELSAGHIER, A ^{[1
]}

MORENO, C ^{[1
]}

IVANYI, J ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,MRC,TB & RELATED INFECT UNIT,DUCANE RD,LONDON W12 0HS,ENGLAND

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1992年 / 35卷 / 06期

关键词：

D O I：

10.1111/j.1365-3083.1992.tb02979.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An immunodominant T-cell-stimulatory epitope located near the carboxy terminus of the 38-kDa antigen from M. tuberculosis (38.G, residues 350-369) was found to be M. tuberculosis-complex specific. This was demonstrated by the presence of proliferative and delayed type hypersensitivity (DTH) responses in mice immunized with Mycobacterium tuberculosis and Mycobacterium bovis BCG, whereas mice immunized with M. avium or other non-tuberculous species of mycobacteria showed no such responses. Peptide 38.G stimulated the proliferation of peripheral blood lymphocytes from healthy purified protein derivative (PPD)-positive individuals, but not from PPD-negative individuals. It also elicited DTH responses in M. tuberculosis sensitized mice and in PPD-positive healthy human volunteers. Peptide 38.G could therefore prove to be an important component in any new molecularly defined reagent used in the immunodiagnosis of tuberculous infection.

引用

页码：711 / 718

页数：8

共 44 条

[1] INTERSPECIES REACTIVITY OF 5 MONOCLONAL-ANTIBODIES TO MYCOBACTERIUM-TUBERCULOSIS AS EXAMINED BY IMMUNOBLOTTING AND ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].