ALDOSTERONE ACTIONS ON BASOLATERAL NA+/H+ EXCHANGE IN MADIN-DARBY CANINE KIDNEY-CELLS

被引:40
作者
VILELLA, S [1 ]
GUERRA, L [1 ]
HELMLEKOLB, C [1 ]
MURER, H [1 ]
机构
[1] UNIV ZURICH,DEPT PHYSIOL,CH-8057 ZURICH,SWITZERLAND
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1992年 / 422卷 / 01期
关键词
ALDOSTERONE; NA+/H+ EXCHANGE; MDCK CELLS;
D O I
10.1007/BF00381507
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
In recent studies, there has been a re-evaluation of the polarity of Na+/H+ exchange in Madin-Darby canine kidney (MDCK) cells. This study was designed to examine aldosterone actions on basolaterally located Na+/H+ exchange of MDCK cell monolayers grown on permeant filter supports; pH(i) was analysed in the absence of bicarbonate by using the pH-sensitive fluorescent probe 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. Pre-exposure of MDCK cells to aldosterone led within 10-20 min to an alkalization of pH(i) (almost-equal-to 0.3 pH unit); this effect is prevented by an addition of dimethylamiloride to the basolateral superfusate. Addition of aldosterone led to stimulation of the basolaterally located Na+/H+ exchange activity (Na+-dependent recovery from an acid load); this effect required preincubation (more then 3 min) and was observed at 0.1 nM aldosterone. Pre-exposure (15 min) of MDCK monolayers to phorbol 12-myristate 13-acetate also led to an activation of Na+/H+ exchange; pre-exposure to 8-bromo-cAMP led to inhibition of Na+/H+ exchange activity. An inhibitory effect of aldosterone was observed if Na+/H+ exchange activity was analysed in the presence of aldosterone; the highest inhibitory effects (20%-30%) occurred at concentrations of 5 nM and higher. Aldosterone-dependent inhibition does not require preincubation and is fully reversible; it was only observed at low (20 mM) but not at high Na+ concentrations (130 mM). The data suggest that aldosterone has an instantaneous inhibitory effect on basolaterally located Na+/H+ exchange activity under conditions of low Na+, but stimulates the rate of transport activity upon preincubation under conditions of physiological Na+ concentrations.
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收藏
页码:9 / 15
页数:7
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