THE AZOTOBACTER-VINELANDII RECA GENE - SEQUENCE-ANALYSIS AND REGULATION OF EXPRESSION

被引：6

作者：

VENKATESH, TV ^{[1
]}

DAS, HK ^{[1
]}

机构：

[1] JAWAHARLAL NEHRU UNIV,CTR BIOTECHNOL,GENET ENGN UNIT,NEW DELHI 110067,INDIA

来源：

GENE | 1992年 / 113卷 / 01期

关键词：

RECOMBINANT DNA; BETA-GALACTOSIDASE ASSAY; LEXA; LACZ FUSION; PROMOTER; SOS INDUCTION;

D O I：

10.1016/0378-1119(92)90668-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequence of the Azotobacter vinelandii recA gene (Av-recA) was determined and compared with the recA sequences from Pseudomonas aeruginosa (Pa-recA), a soil bacterium. and Escherichia coli (Ec-recA), an enteric bacterium. The Av-recA gene and the deduced aa sequence were found to be more similar to their Pa-recA counterparts than to the Ec-recA gene and protein. Expression of Av-recA was found to be autoregulatory. Unlike Ec-recA and Pa-recA. however, expression of Av-recA was weakly enhanced upon DNA damage. In E. coli, expression of an Av-recA = lacZ fusion was poor, but its autoregulation was similar to that of Ec-recA. Av-recA expression, however, could not induce the repair system response in E. coli.

引用

页码：47 / 53

页数：7

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