ANALYSIS OF ELECTROPHORETIC WHOLE-CELL PROTEIN PROFILES AS A TOOL FOR CHARACTERIZATION OF ENTEROCOCCUS SPECIES

被引：49

作者：

MERQUIOR, VLC

PERALTA, JM

FACKLAM, RR

TEIXEIRA, LM

机构：

[1] UNIV FED RIO DE JANEIRO,INST MICROBIOL,BR-21941 RIO JANEIRO,BRAZIL

[2] CTR DIS CONTROL & PREVENT,RESP DIS BRANCH,ATLANTA,GA

来源：

CURRENT MICROBIOLOGY | 1994年 / 28卷 / 03期

关键词：

D O I：

10.1007/BF01571056

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The whole-cell protein-profiling technique was evaluated for identifying Enterococcus species. Reference strains, strains from human infections, from animals other than human, and from environmental sources were studied. Whole-cell extracts were obtained by lysozyme treatment and were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and densitometry. Each Enterococcus species had a unique and distinguishable whole-cell protein profile. The major differences among species-specific profiles were found in the positions corresponding to 60-40 and 30-20 kDa. Profiles of the same species did not show qualitative variations. Analysis of whole-cell protein profiles was shown to be a relatively simple, easy, and reproducible procedure for the reliable and fast differentiation and identification of the enterococcal species.

引用

页码：149 / 153

页数：5

共 32 条

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