FURTHER IDENTIFICATION OF BIOACTIVE PEPTIDES IN THE ANTERIOR BYSSUS RETRACTOR MUSCLE OF MYTILUS - 2 CONTRACTILE AND 3 INHIBITORY PEPTIDES

被引:13
作者
FUJISAWA, Y
TAKAHASHI, T
IKEDA, T
MUNEOKA, Y
KUBOTA, I
MINAKATA, H
NOMOTO, K
NOSE, T
MIKI, W
机构
[1] HIROSHIMA UNIV, FAC INTEGRATED ARTS & SCI, HIROSHIMA 724, JAPAN
[2] SUNTORY BIOPHARMA TECH CTR, GUNMA 37005, JAPAN
[3] SUNTORY INST BIOORGAN RES, OSAKA 618, JAPAN
[4] MARINE BIOTECHNOL INST, SHIMIZU 424, JAPAN
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 1993年 / 106卷 / 01期
关键词
D O I
10.1016/0742-8413(93)90281-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
1. Two contractile and three inhibitory peptides were newly isolated from the anterior byssus retractor muscles (ABRMS) of the bivalve mollusc Mytilus edulis by using the muscle as the bioassay system. 2. The structures of the two contractile peptides were GPFGTHKamide (GPFG-8) and GPFGLNKHGamide (GPFG-9). The contractile response of the ABRM to the first-time application of GPFG-8 or GPFG-9 was of considerable size. The threshold concentrations of the peptides were around 10(-9) M. However, the contractile response to the second-time application was far smaller than that to the first-time application in both cases. Namely, the muscle showed tachyphylaxis to the peptides. 3. Two of the three inhibitory peptides were members of the Mytilus-inhibitory-peptide (MIP) family. Their structures were RAPLFIamide (MIP6) and RSPMFVamide (MIP7). The peptides, as well as the other MIPs previously identified, showed a potent inhibitory effect on phasic contraction of the ABRM in response to repetitive electrical stimulation. The remaining one was an MIP-related peptide (MIP-RP) having the sequence of MRYFVamide. The MIP-RP was less potent than the two MIPs in inhibiting the contraction of the ABRM.
引用
收藏
页码:261 / 267
页数:7
相关论文
共 14 条
[1]  
FUJISAWA Y, 1990, PEPTIDE CHEMISTRY 1989, P51
[2]  
FUJISAWA Y, 1991, COMP BIOCHEM PHYS C, V100, P525
[3]   THE FMRFAMIDE-RELATED DECAPEPTIDE OF MYTILUS CONTAINS A D-AMINO-ACID RESIDUE [J].
FUJISAWA, Y ;
IKEDA, T ;
NOMOTO, K ;
YASUDAKAMATANI, Y ;
MINAKATA, H ;
KENNY, PTM ;
KUBOTA, I ;
MUNEOKA, Y .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1992, 102 (01) :91-95
[4]   A MYTILUS PEPTIDE RELATED TO THE SMALL CARDIOACTIVE PEPTIDES (SCPS) - STRUCTURE DETERMINATION AND PHARMACOLOGICAL CHARACTERIZATION [J].
FUJISAWA, Y ;
KUBOTA, I ;
NOMOTO, K ;
MINAKATA, H ;
YASUDAKAMATANI, Y ;
IKEDA, T ;
MUNEOKA, Y .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1993, 104 (03) :469-475
[5]  
FUJISAWA Y, 1991, STUD NEUROS, V11, P97
[6]   STRUCTURES AND ACTIONS OF MYTILUS INHIBITORY PEPTIDES [J].
HIRATA, T ;
KUBOTA, I ;
IWASAWA, N ;
TAKABATAKE, I ;
IKEDA, T ;
MUNEOKA, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 152 (03) :1376-1382
[7]   CATCH-RELAXING PEPTIDE ISOLATED FROM MYTILUS PEDAL GANGLIA [J].
HIRATA, T ;
KUBOTA, I ;
TAKABATAKE, I ;
KAWAHARA, A ;
SHIMAMOTO, N ;
MUNEOKA, Y .
BRAIN RESEARCH, 1987, 422 (02) :374-376
[8]   MYTILUS-INHIBITORY PEPTIDE ANALOGS ISOLATED FROM THE GANGLIA OF A PULMONATE MOLLUSK, ACHATINA-FULICA [J].
IKEDA, T ;
YASUDAKAMATANI, Y ;
MINAKATA, H ;
KENNY, PTM ;
NOMOTO, K ;
MUNEOKA, Y .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1992, 101 (02) :245-249
[9]  
IKEDA T, 1991, PEPTIDE CHEMISTRY 1990, P357
[10]  
IKEDA T, 1993, IN PRESS PEPTIDE CHE