A QUANTITATIVE BIOASSAY SPECFIC FOR POLYGALACTURONASES

被引：19

作者：

MUSSELL, HW

MORRE, DJ

机构：

[1] Department of Botany and Plant Pathology, Purdue University, Lafayette, IN

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90190-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A bioassay for PG activity based on fresh weight loss of cucumber pericarp tissue is described which is specific, quantitative, sensitive and reproducible. The assay is at least 500 times more sensitive than previously described techniques. The assay is unaffected by nonpectolytic hydrolases (including pectin esterase), pectin, or polygalacturonic acid and requires no purification of test extracts. © 1969.

引用

页码：353 / &

共 8 条

[1]

BATEMAN DF, 1965, PHYTOPATHOLOGY, V55, P204

[2]

BATEMAN DURWARD F., 1966, ANNU REV PHYTOPATHOL, V4, P119, DOI 10.1146/annurev.py.04.090166.001003

[3] REGULATION OF INDUCED CELLULASE SYNTHESIS IN PYRENOCHAETA TERRESTRIS GORENZ ET AL. BY UTILIZABLE CARBON COMPOUNDS [J].