ISOLATION AND CHEMICAL CHARACTERIZATION OF 2 DISTINCT LINK PROTEINS FROM BOVINE NASAL CARTILAGE PROTEOGLYCAN COMPLEX

被引:60
作者
BONNET, F
PERIN, JP
JOLLES, P
机构
[1] Laboratory of Proteins, University of Paris V, F-75270 Paris Cedex 06
关键词
D O I
10.1016/0005-2795(78)90578-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bovine nasal cartilage proteoglycan aggregates have been dissociated and separated by dissociative density gradient centrifugation into proteoglycan subunits and link fraction". The latter contained mainly the two "link proteins" as shown by analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The two "link proteins" were then separated by preparative gel electrophoresis under dissociative conditions. Molecular weight and amino acid composition of both proteins are presented. © 1978."
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页码:242 / 248
页数:7
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