EXPRESSION OF CLASS-II MOLECULES ON INTESTINAL EPITHELIAL-CELLS IN HUMANS - DIFFERENCES BETWEEN NORMAL AND INFLAMMATORY BOWEL-DISEASE

被引:239
作者
MAYER, L
EISENHARDT, D
SALOMON, P
BAUER, W
PLOUS, R
PICCININI, L
机构
[1] MT SINAI MED CTR,DIV ENDOCRINOL,NEW YORK,NY 10029
[2] MT SINAI MED CTR,DEPT PATHOL,NEW YORK,NY 10029
关键词
D O I
10.1016/0016-5085(91)90575-6
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Expression of class II antigens on human intestinal epithelial cells was assessed using a sensitive avidinbiotin-peroxidase technique. HLA-DR was present predominantly in the normal small bowel with diminished but evident expression in the colon. HLA-DP staining was less prominent, and HLA-DQ was absent. In inflammatory bowel disease the expression of both HLA-DR and HLA-DP was increased, but that for HLA-DQ remained absent, suggesting an inherent defect in the ability of intestinal epithelial cells to express HLA-DQ. In related experiments, an interferon gamma-treated malignant epithelial cell line T84 also failed to stain for HLA-DQ and HLA-DP despite the presence of HLA-DR. Isolated RNAs for all three subclasses of HLA-D were detectable by slot-blot analysis, suggesting that the lack of HLA-DQ expression relates to posttran-scriptional defects in intestinal epithelium. These and other differences with conventional class II antigen-positive accessory cells (macrophages/B cells) may help to explain the unique properties of intestinal epithelial cells as antigen-presenting cells. © 1991 by the American Gastroenterological Association.
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页码:3 / 12
页数:10
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