QUANTITATIVE-ANALYSIS OF THE PURKINJE-CELL POPULATION DURING EXTREME AGING IN THE CEREBELLUM OF THE WISTAR LOUVAIN RAT

被引:27
作者
BAKALIAN, A
CORMAN, B
DELHAYEBOUCHAUD, N
MARIANI, J
机构
[1] UNIV PARIS 06,NEUROBIOL & DEV LAB,F-75230 PARIS 05,FRANCE
[2] CENS,DEPT BIOL,BIOL CELLULAIRE LAB,F-91191 GIF SUR YVETTE,FRANCE
关键词
AGING; CEREBELLUM; PURKINJE CELLS; CELL COUNTS; WISTAR LOUVAIN RAT;
D O I
10.1016/0197-4580(91)90068-U
中图分类号
R592 [老年病学]; C [社会科学总论];
学科分类号
03 ; 0303 ; 100203 ;
摘要
The loss of neurons is viewed as one of several causes of the deterioration of neural function during ageing. However, the existing experimental evidence for an age-related decrease in the neuronal number may be misinterpreted due to the way the cells are counted and to the interference of unsuspected degenerative pathology of the animals studied. To reinvestigate this question we have quantified as easily identifiable population of neurons, the cerebellar Purkinje cells, in very old but healthy rats. The number of Purkinje cells in the cerebellum was assessed in two populations of rats: control (10 months) and old (42 months) rats from the Wistar/Louvain strain. In both groups, paraffin-embedded brains were cut serially in the sagittal plane. Purkinje cells were counted every 15 or 22 sections under the light microscope at a magnification of 1250 x. The raw value of cell counts were corrected according to the method of Hendry (21) in order to avoid the overestimation due to splitting of the nucleus during sectioning. The latero-lateral extent of the cerebellar cortex, obtained by multiplying the thickness of the section by the number of sections in which Purkinje cells were counted, was not statistically different (mean +/- standard deviation): 12.8 +/- 1.16 mm (n = 6) for the control rats and 12.0 +/- 1.02 mm for the old animals (n = 8) (Student's t-test, p = 0.18). The corrected number of the Purkinje cells (mean +/- standard deviation) was 330,350 +/- 35,448 cells (n = 6) for the control animals and 299,019 +/- 50,223 (n = 8) cells for the old rats. The 9.5% reduction observed in aged animals was not statistically significant (p = 0.22). In conclusion, our studies indicate that, in the cerebellum of the Wistar/Louvain rat, if anything, the effect of ageing upon the Purkinje cell population is very mild.
引用
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页码:425 / 430
页数:6
相关论文
共 46 条
[1]  
ARMSTRONG TM, 1970, J COMP NEUROL, V139, P449
[2]   INTERACTION OF NOREPINEPHRINE WITH PURKINJE-CELL RESPONSES TO CEREBELLAR AFFERENT INPUTS IN AGED RATS [J].
BICKFORD, PC ;
HOFFER, BJ ;
FREEDMAN, R .
NEUROBIOLOGY OF AGING, 1985, 6 (02) :89-94
[3]   A QUALITATIVE AND QUANTITATIVE LIGHT MICROSCOPIC STUDY OF THE INFERIOR OLIVARY COMPLEX IN THE ADULT STAGGERER MUTANT MOUSE [J].
BLATT, GJ ;
EISENMAN, LM .
JOURNAL OF NEUROGENETICS, 1985, 2 (01) :51-66
[4]   THE IMPACT OF RECENT STEREOLOGICAL ADVANCES ON QUANTITATIVE STUDIES OF THE NERVOUS-SYSTEM [J].
BRAENDGAARD, H ;
GUNDERSEN, HJG .
JOURNAL OF NEUROSCIENCE METHODS, 1986, 18 (1-2) :39-78
[5]   MORPHOLOGICAL OBSERVATIONS ON THE CEREBELLAR CORTEX [J].
BRAITENBERG, V ;
ATWOOD, RP .
JOURNAL OF COMPARATIVE NEUROLOGY, 1958, 109 (01) :1-33
[7]   STRUCTURAL AND QUANTITATIVE STUDIES ON THE NORMAL C3H AND LURCHER MUTANT MOUSE [J].
CADDY, KWT ;
BISCOE, TJ .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1979, 287 (1020) :167-&
[8]  
DAMBIERMONTMONA.R, 1974, J COMP NEUROL, V155, P61
[9]  
DELORENZI ENZO, 1931, ZEITSCHR WISS BIOL ABT B ZEITSCHR ZELLFORSCH U MIKROSK ANAT, V14, P310, DOI 10.1007/BF00397326
[10]  
DELORENZI ENZO, 1931, BOLL SOC ITAL BIOL SPERIM, V6, P80