NUCLEOTIDE-SEQUENCES OF GENES ENCODING PENICILLIN-BINDING PROTEINS FROM STREPTOCOCCUS-PNEUMONIAE AND STREPTOCOCCUS-ORALIS WITH HIGH HOMOLOGY TO ESCHERICHIA-COLI PENICILLIN-BINDING PROTEINS 1A AND 1B

被引:69
作者
MARTIN, C [1 ]
BRIESE, T [1 ]
HAKENBECK, R [1 ]
机构
[1] MAX PLANCK INST MOLEC GENET, IHNESTR 73, W-1000 BERLIN 33, GERMANY
关键词
D O I
10.1128/JB.174.13.4517-4523.1992
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nucleotide sequence of a 3,378-bp DNA fragment of Streptococcus pneumoniae that included the structural gene for penicillin-binding protein (PBP) 1a (ponA), which encodes 719 amino acids, was determined. Homologous DNA fragments from an S. oralis strain were amplified with ponA-specific oligonucleotides. The 2,524-bp S. oralis sequence contained the coding region for the first 636 amino acids of a PBP. The coding sequence differed by 437 nucleotides (27%) and one additional triplet, resulting in 87 amino acid substitutions (14%), from S. pneumoniae PBP 1a. Both PBPs are highly homologous to bifunctional high-M(r) Escherichia coli PBPs 1a and 1b.
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页码:4517 / 4523
页数:7
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