NUCLEOTIDE-SEQUENCE OF A CDNA FOR CANINE AMYLIN

被引：4

作者：

ALBRANDT, K

MULL, E

COOPER, GJS

JOHNSON, MJ

机构：

[1] Department of Molecular Biology, Amylin Corporation, San Diego, CA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1130卷 / 01期

关键词：

DIABETES-MELLITUS; AMYLOIDOGENIC; AMINO ACID SEQUENCE; (DOG);

D O I：

10.1016/0167-4781(92)90470-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Canine amylin cDNA was cloned and sequenced from a dog pancreas library using oligonucleotide probes corresponding to portions of human amylin. The sequence reported here is significantly different from an incomplete sequence previously reported. The non-amyloidogenic canine mature peptide is 89% and 95% identical to its amyloidogenic human and cat homologues, respectively. Both the dog and cat peptides are identical over the proposed amyloidogenic region, amino acids 20-29. Hydropathy plots comparing these three peptides are nearly identical suggesting that the primary sequence of amylin is in itself insufficient to explain pancreatic amyloid formation.

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页码：97 / 99

页数：3

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