DIRECT SELECTION OF DNA-SEQUENCES CONSERVED BETWEEN SPECIES

被引：17

作者：

SEDLACEK, Z ^{[1
]}

KONECKI, DS ^{[1
]}

SIEBENHAAR, R ^{[1
]}

KIOSCHIS, P ^{[1
]}

POUSTKA, A ^{[1
]}

机构：

[1] DEUTSCH KREBSFORSCHUNGSZENTRUM,NEUENHEIMER FELD 506,W-6900 HEIDELBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 15期

关键词：

D O I：

10.1093/nar/21.15.3419

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An essential requirement in the analysis of genomes is the identification of functionally important sequence elements, which are often evolutionarily conserved. We describe here the development of a novel procedure for the selective isolation of conserved sequences which is based on hybridization of PCR-amplifiable DNA fragments from the whole genome of one species to biotinylated DNA from a genomic region of another species. The interspecies DNA hybrids are immobilized and the PCR-amplifiable DNA fragments are eluted, amplified and after further hybridization-amplification rounds cloned. This method was used for the generation of sublibraries of conserved sequences from mouse and pig DNA from regions corresponding to cosmids from the human Xq28 region. Mouse and pig homologs of sequences containing exons of known human genes, as well as exons from novel genes have been identified.

引用

页码：3419 / 3425

页数：7

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