AN NAD DERIVATIVE PRODUCED DURING TRANSFER-RNA SPLICING - ADP-RIBOSE 1''-2'' CYCLIC PHOSPHATE

被引：88

作者：

CULVER, GM

MCCRAITH, SM

ZILLMANN, M

KIERZEK, R

MICHAUD, N

LAREAU, RD

TURNER, DH

PHIZICKY, EM

机构：

[1] UNIV ROCHESTER,SCH MED & DENT,DEPT BIOCHEM,601 ELMWOOD AVE,ROCHESTER,NY 14642

[2] POLISH ACAD SCI,INST BIOORGAN CHEM,PL-60704 POZNAN,POLAND

[3] UNIV ROCHESTER,DEPT BIOL,ROCHESTER,NY 14627

[4] UNIV ROCHESTER,DEPT CHEM,ROCHESTER,NY 14627

来源：

SCIENCE | 1993年 / 261卷 / 5118期

关键词：

D O I：

10.1126/science.8392224

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transfer RNA (tRNA) splicing is essential in Saccharomyces cerevisiae as well as in humans, and many of its features are the same in both. In yeast, the final step of this process is removal of the 2' phosphate generated at the splice junction during ligation. A nicotinamide adenine dinucleotide (NAD)-dependent phosphotransferase catalyzes removal of the 2' phosphate and produces a small molecule. It is shown here that this small molecule is an NAD derivative: adenosine diphosphate (ADP)-ribose 1''-2'' cyclic phosphate. Evidence is also presented that this molecule is produced in Xenopus laevis oocytes as a result of dephosphorylation of ligated tRNA.

引用

页码：206 / 208

页数：3

共 39 条

[1] PARTICIPATION OF THE INTRON IN THE REACTION CATALYZED BY THE XENOPUS TRANSFER-RNA SPLICING ENDONUCLEASE [J].